5-7 Examples of growth curve data and graphs

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The data shown here was generated by growing E. coli in four different media and then comparing the results. Before you do anything, think about the various media and hypothesize about which condition would have the fastest growth rate. Then, use this data to prepare graphs of the four conditions. Once created, choose appropriate points and determine the growth rate and generation time of each culture.

Growth of E. coli in minimal medium + lactose at 37 °C.

Time (hours) VPC1 VPC2
7.80 x 106
1.01.08 x 107
2.03.20 x 107
2.56.40 x 107
3.02.02 x 108
3.53.56 x 108
4.09.60 x 1084.1 x 108
4.53.60 x 108
5.09.50 x 1082.50 x 108
5.51.83 x 1091.07 x 109
6.01.05 x 109
6.51.41 x 109
7.01.03 x 10100
7.51.00 x 109
24.54.20 x 108
25.51.00 x 108
26.57.00 x 108

E. coli grown in Minimal medium + glucose

Time VPC1 VPC2
0.0 1.20 x 107
0.5 1.13 x 107
1.0 1.03 x 1071.40 x 107
1.5 2.48 x 107 2.10 x 107
2.0 6.00 x 107
2.5 8.40 x 107
3.0 1.66 x 108
3.5 3.30 x 108
4.0 2.90 x 109 1.80 x 109
4.5 6.20 x 108 9.40 x 108
5.0 7.50 x 108 1.09 x 109
5.5 2.00 x 109 1.60 x 109
6.0 1.70 x 109 2.42 x 109
6.5 2.00 x 109 1.48 x 109
7.5 3.48 x 109
8.0 4.00 x 109
12.0 4.10 x 109
16.0 3.70 x 109
20.0 8.00 x 108
24.5 1.25 x 108
26.5 1.23 x 108

E. coli grown in Minimal medium + glycerol

TimeVPC1VPC2
0.0 2.52 x 107
0.5 1.11 x 107
1.0 9.80 x 106
1.5 1.96 x 107
2.0 1.65 x 107
2.5 3.80 x 107
3.0 4.10 x 107
3.5 9.10 x 107
4.0 5.80 x 107 1.50 x 108
4.5 2.23 x 107
5.0 2.23 x 107 4.10 x 108
5.5 6.20 x 108 5.10 x 108
6.0 5.00 x 108 7.10 x 108
6.5 1.16 x 108 2.48 x 108
7.5 1.90 x 108
24.5 3.56 x 108
25.5 8.60 x 108 4.10 x 108
26.5 6.30 x 108 6.20 x 108

E. coli grown in Rich medium (Nutritent Broth + Glucose + Yeast Extract)

Time (hours) VPC1 VPC2
0.0
0.5 5.01 x 106
1.0 4.90 x 106
1.5 5.20 x 106
2.0 1.46 x 108
2.5 8.35 x 107
3.0 3.85 x 108
3.5 5.50 x 108
4.0 5.55 x 108
4.5 9.10 x 108
5.0 8.40 x 108
5.5 9.10 x 108
6.0 1.10 x 109
6.5 3.90 x 109 7.00 x 108
7.0 7.40 x 108
7.5 7.70 x 108
25.5 5.00 x 108
26.5 7.70 x 108

After looking at the data and determining your growth rates, did your actual results match your hypothesis? In either case explain your reasoning.

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